The Aldol addition has been a staple in a chemist education since the 1870’s, but finding an enzyme equivalent has been difficult as the main biological aldoses will not stop the reaction at the 1st

A client tasked EnzBond with re-evolving the aldose to perform the single step-aldol and stop there. The client had no data to support the evolution campaign but EnzBond was able to design 20 novel variants in one round with: